Effects of Rotenone and Cyanide on the ETC

The Electron Transport Chain

 

Rotenone and Cyanide are both inhibitors of the ETC that prevent the production of the proton mtive force (PMF) that allows for the formation of ATP from ADP and an inorganic Phosphate group.

Cyanide binds to the Iron(III) in the Heme group of the Cytochrom oxidase C which is associated with Complex IV. The binding of cyanie prevents the oxidation reaction from taking place so no energy is released to allow the Complex to pump the Hydrogen ions out. Thus, the PMF does not build up and there is not enough energy to allow the H ions to return via ATP synthase and be coupled to the formation of ATP.

Rotenone works in a similar manner but affects Complex I instead.

Rotenone prevents the use of NAH as a substrate so oxidation of the NADH to NAD+ does not take place. No oxidation = no energy to pump the H ions into the intermembrane space leading to a weak PMF that does is not enough to drive the H+ back into the matrix and be coupled to ATP formation.

 

Therefore, to answer the question posed to us in the tutorial:

Cyanide affects Complex IV

Rotenone affects Complex I

And they both result in your death in high enough doses
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2 Responses to Effects of Rotenone and Cyanide on the ETC

  1. illuminolist says:

    πŸ˜€ @R2D2 πŸ™‚
    great post, amrita

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